JSON RDF ISO19115-3 XML

Detection and quantitation of Saxitoxin and its analogues from strains of the freshwater cyanobacterium, Anabaena circinalis

Created 24/06/2017

Updated 09/10/2017

JSON RDF ISO19115-3 XML

Fourteen isolates of Anabaena circinalis from various regions of Australia were grown for extraction. Two microtiter plate based bioassays for measuring saxitoxin (STX) and its derivatives, use radioactively labeled STX binding by sodium channels, STX's pharmacological target, or an unrelated protein, saxiphilin. These bioassays were challenged with extracts of toxic and nontoxic strains of Anabaena circinalis, and the results were compared with HPLC analysis.

Cultured cells were harvested during late logarithmic growth phase. A saxiphilin microtiter plate binding assay was conducted using tritiated saxitoxin ([3H]STX) from the centipede Ethmostigmus rubripes. The inhibition of [3H]STX binding to rat brain sodium channels was also measured - both values were recorded as percentages.

Samples were screened for inhibition of [3H]STX binding by incubating duplicate samples of 10 µL each. If dilution was necessary, extracts were retested in a series (2-, 5-, 10-, 50-, 100-, 500-, and 1000-fold dilutions). Three classes of paralytic shellfish toxins (PSTs) were analyzed by HPLC - C-toxins, gonyautoxins, and saxitoxins (C1, C2, dcGTX3, GTX5, dcGTX2, GTX3, GTX2, dcSTX, STX; values for individual toxin analogues are given as mol %).

The final toxicity measurement was the average of the values derived from the multiple inhibitory values between 20 and 80% inhibition obtained in the titration of unknown samples.

Files and APIs

Tags

Additional Info

Field Value
Title Detection and quantitation of Saxitoxin and its analogues from strains of the freshwater cyanobacterium, Anabaena circinalis
Language English
Licence Other
Landing Page https://devweb.dga.links.com.au/data/dataset/056593af-2ab1-465a-ab56-f5a21fc54196
Contact Point
Australian Institute of Marine Science
adc@aims.gov.au
Geospatial Coverage GA1
Data Portal data.gov.au

Data Source

This dataset was originally found on data.gov.au "Detection and quantitation of Saxitoxin and its analogues from strains of the freshwater cyanobacterium, Anabaena circinalis". Please visit the source to access the original metadata of the dataset:
https://devweb.dga.links.com.au/data/dataset/detection-and-quantitation-of-saxitoxin-and-its-analogues-from-strains-of-the-freshwater-cyanob

Original dataset
Dataset copies

No duplicate datasets found.