The identification of suitable abiotic surrogates for biological diversity requires the collection of both physical and biological data. However, logistical constraints often preclude experimental designs that incorporate spatial and temporal replication. Given the quite limited resources normally available for surveys, the investigation of appropriate surrogates involves a trade-off between overall spatial coverage and replication. We have completed a survey in Jervis Bay in which environmental and infaunal data were collected contemporaneously in order to be combined with similar data from a previous winter survey (survey number GA309) to investigate variation across seasons. Because there will be a certain error in sampling at the exact location as the previous survey, the survey design also required that replicate samples be taken at a set number of stations in order to investigate fine-scale variability (at the scale of metres).
We used grabs to collect paired geochemical and biological samples from thirty-two stations in a defined grid near Darling Rd; at eight of these stations we deployed three pairs of grabs to investigate fine-scale variability. Due to good weather and extra ship time available, we also deployed a CTD to investigate vertical temperature and salinity profiles at each station in the Darling Rd grid, as well as at stations throughout the entire bay. Samples are expected to be processed and analysed by late 2009, but preliminary results indicate that most physical variables and infaunal assemblages varied between seasons. In addition, variation among infaunal assemblages seems greater among stations (hundreds of meters) than within replicates at stations (meters).