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The behaviour of mixtures of paralytic shellfish toxins in receptor dependent assays

Created 24/06/2017

Updated 09/10/2017

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Using two unrelated STX-binding proteins (the rat brain Na channel and a saxiphilin from the xanthid crab Liomera tristis), this study experimentally validated the predicted combined effects of binary and ternary mixtures of paralytic shellfish toxins (PSTs).

All toxin dilutions (tritiated saxitoxin ([3H]STX), STX dihydrochloride, neoSTX, dcSTX, and GTX5) were in 0.01 M acetic acid. The L. tristis saxiphilin assay was conducted in high protein binding 96 well filtration plates and the rat brain Na channel binding of [3H]STX was measured using a microtiter plate method.

The model predicts the amount of inhibition by toxin mixtures in competitive binding assays from which an IC50 (concentration that inhibits radioligand binding by 50%) can be calculated. IC50 values and their 95% confidence limits from experimental data were derived using the single site curve equation of Graphpad Prism (Ver 4.0, Graphpad Software, San Diego, CA), with fitting constrained to a slope of 1 and a baseline of 0.

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Field Value
Title The behaviour of mixtures of paralytic shellfish toxins in receptor dependent assays
Language English
Licence Other
Landing Page https://devweb.dga.links.com.au/data/dataset/40469c52-b23a-46e4-b8c0-454248800125
Contact Point
Australian Institute of Marine Science
adc@aims.gov.au
Geospatial Coverage GA1
Data Portal data.gov.au

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This dataset was originally found on data.gov.au "The behaviour of mixtures of paralytic shellfish toxins in receptor dependent assays". Please visit the source to access the original metadata of the dataset:
https://devweb.dga.links.com.au/data/dataset/the-behaviour-of-mixtures-of-paralytic-shellfish-toxins-in-receptor-dependent-assays

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