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Bacterial communities using 16S rDNA from calcareous sediments of nearshore and outer shelf reefs, Great Barrier Reef

Created 24/06/2017

Updated 09/10/2017

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Bacterial communities in eight 16S rDNA clone libraries from calcareous sediments were investigated to assess bacterial diversity in sediments of the Great Barrier Reef and to investigate differences due to decreased water quality. Samples were taken from 2 locations (fore and back reefs) on each of 4 coral reefs: nearshore (Fitzroy and High Islands, subject to enhanced runoff) on the outer shelf (Hastings and Flynn Reefs, pristine conditions).

Out of 221 non-chimeric sequences, 189 (85.5%) were unique and only one sequence occurred in more than one library. Cluster analyses and comparison to published sequences indicated that sequences retrieved belonged to the alpha, gamma and delta subdivisions of the Proteobacteria; Cytophaga, Flavobacteria, Bacteroidetes (CFB) group; Cyanobacteria; Planctomycetaceae; Verrucomicrobiaceae; and Acidobacteriaceae.

Carbon (organic carbon, total carbon) and nitrogen in the sediments were analysed from two additional samples from each location. Calcium carbonate (%), exposure (front=more exposed to wave action; backreef=less exposure) and mean grain size (mm) were also recorded.

Neighbour-joining trees were constructed representing sequences from the 8 clone libraries.

All data sequence data are deposited on Genbank (DQ256505 to DQ256725).

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Title Bacterial communities using 16S rDNA from calcareous sediments of nearshore and outer shelf reefs, Great Barrier Reef
Language English
Licence Other
Landing Page https://devweb.dga.links.com.au/data/dataset/6d4ee133-b9f4-48c3-a60f-802027e7bc5f
Contact Point
Australian Institute of Marine Science
adc@aims.gov.au
Geospatial Coverage {"type": "Polygon", "coordinates": [[[145.95, -17.2], [146.3, -17.2], [146.3, -16.5], [145.95, -16.5], [145.95, -17.2]]]}
Data Portal data.gov.au

Data Source

This dataset was originally found on data.gov.au "Bacterial communities using 16S rDNA from calcareous sediments of nearshore and outer shelf reefs, Great Barrier Reef". Please visit the source to access the original metadata of the dataset:
https://devweb.dga.links.com.au/data/dataset/bacterial-communities-using-16s-rdna-from-calcareous-sediments-of-nearshore-and-outer-shelf-ree

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No duplicate datasets found.