From Australian Oceans Data Network
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Distribution of Vitamin B12 in waters of the central Great Barrier Reef

Created 12/03/2025

Updated 12/03/2025

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A highly sensitive and selective method was used to estimate concentrations of cyanocobalamin (Vitamin B12) in sea water along a transect of reefs across the central portion of the Great Barrier Reef. Water samples were collected from a depth of 0.5 m along each transect (moving from reef front to reef back) at Pandora, Rib and Myrmidon Reefs. Samples were also collected from Palm Passage at a depth of 10m. Analyses were based on clinical techniques of radioimmunoassay, combining the RIA method with preconcentration of Vitamin B12 by displacement liquid chromatography on C18 SEP-PAK columns. Analyses of the column eluate by radioassay proved sensitive to B12 at femtomolar concentrations (50-2400 pg B12/L seawater). The concentrations calculated from the radioassay translated directly into units of pg B12/L and ng folate/L seawater as a consequence of the concentration procedure. Concentrations above calibration range (2400 pg B 12; 24.0 ng folate) were determined by extrapolation. To determine changes in Vitamin B12 concentrations of sea water along a transect of reefs across the central portion of the Great Barrier Reef.

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Field Value
Title Distribution of Vitamin B12 in waters of the central Great Barrier Reef
Language eng
Licence notspecified
Landing Page https://devweb.dga.links.com.au/data/dataset/8316ef39-dcde-4942-baf7-6b96220e0068
Contact Point
CSIRO Oceans & Atmosphere
reception@aims.gov.au
Reference Period 20/11/2017
Geospatial Coverage {"type": "Point", "coordinates": [147.389124, -18.266752]}
Data Portal data.gov.au

Data Source

This dataset was originally found on data.gov.au "Distribution of Vitamin B12 in waters of the central Great Barrier Reef". Please visit the source to access the original metadata of the dataset:
https://devweb.dga.links.com.au/data/dataset/distribution-of-vitamin-b12-in-waters-of-the-central-great-barrier-reef1

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