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A new measure of particulate polysaccharides on the Great Barrier Reef

Created 24/06/2017

Updated 09/10/2017

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A test using fluorescently labeled concanavalin A (FITC-conA), a lectin (a group of proteins that bind polysaccharides), was used to measure particulate polysaccharides (PPs) at 11 stations on the Great Barrier Reef. In conjunction, 96-well filtration plate technology was used to increase throughput of samples.

The experiment tested a number of factors: 5 different concentrations of FITC-conA (5, 10, 20, 30, 40 µg mL-1); differences in average fluorescence (over 4 replicates) of samples using filtered and unfiltered FITC-conA; the effect of increasing incubation period of FITC-conA on fluorescence intensity (for both seasons). Concentrations of transparent exopolymer particles (TEPs) from each site in the wet season were measured using the Alcian blue technique for comparison and verification of levels. PP concentrations were compared to concentrations of chlorophyll a, pheophytin and particulate nitrogen to assess phytoplankton as a possible source of PPs.

Samples were collected from each station in the wet (February 2006) and dry seasons (August 2006). Other station parameters recorded were: Secchi depth; total suspended solids (TSS); particulate organic carbon (POC); particulate phosphorous (PP); particulate nitrogen (PN); particulate polysaccharides (PPs); phaeophytin; chlorophyll a.

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Title A new measure of particulate polysaccharides on the Great Barrier Reef
Language English
Licence Other
Landing Page https://devweb.dga.links.com.au/data/dataset/b9b82b52-c7b3-4523-bda1-1dd073cd0693
Contact Point
Australian Institute of Marine Science
adc@aims.gov.au
Geospatial Coverage {"type": "Polygon", "coordinates": [[[148.85, -20.62], [149.22, -20.62], [149.22, -19.8], [148.85, -19.8], [148.85, -20.62]]]}
Data Portal data.gov.au

Data Source

This dataset was originally found on data.gov.au "A new measure of particulate polysaccharides on the Great Barrier Reef". Please visit the source to access the original metadata of the dataset:
https://devweb.dga.links.com.au/data/dataset/a-new-measure-of-particulate-polysaccharides-on-the-great-barrier-reef

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