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Distribution of Vitamin B12 in waters of the central Great Barrier Reef

Created 24/06/2017

Updated 09/10/2017

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A highly sensitive and selective method was used to estimate concentrations of cyanocobalamin (Vitamin B12) in sea water along a transect of reefs across the central portion of the Great Barrier Reef. Water samples were collected from a depth of 0.5 m along each transect (moving from reef front to reef back) at Pandora, Rib and Myrmidon Reefs. Samples were also collected from Palm Passage at a depth of 10m.

Analyses were based on clinical techniques of radioimmunoassay, combining the RIA method with preconcentration of Vitamin B12 by displacement liquid chromatography on C18 SEP-PAK columns. Analyses of the column eluate by radioassay proved sensitive to B12 at femtomolar concentrations (50-2400 pg B12/L seawater). The concentrations calculated from the radioassay translated directly into units of pg B12/L and ng folate/L seawater as a consequence of the concentration procedure. Concentrations above calibration range (2400 pg B 12; 24.0 ng folate) were determined by extrapolation.

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Title Distribution of Vitamin B12 in waters of the central Great Barrier Reef
Language English
Licence Other
Landing Page https://devweb.dga.links.com.au/data/dataset/a7c39ae2-5605-4fb1-8d48-019a6b2bf11c
Contact Point
Australian Institute of Marine Science
adc@aims.gov.au
Geospatial Coverage {"type": "Point", "coordinates": [147.389124, -18.266752]}
Data Portal data.gov.au

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This dataset was originally found on data.gov.au "Distribution of Vitamin B12 in waters of the central Great Barrier Reef". Please visit the source to access the original metadata of the dataset:
https://devweb.dga.links.com.au/data/dataset/distribution-of-vitamin-b12-in-waters-of-the-central-great-barrier-reef

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