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Antioxidant activity in extracts of marine organisms screened by microtiter plate assay

Created 24/06/2017

Updated 09/10/2017

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A novel microtiter plate assay was developed to determine the total peroxyl radical-trapping activity of antioxidants extracted from marine organisms by measuring the inhibition rate of dye-substrate oxidation. The 96-well microtiter plate format afforded multisample throughput.

Dihydrorhodamine-123 (DHR), dihydrofluorescein (DHR), and dichlorodihydrofluorescein (DCDHF) were used as reduced substrates for oxidation by peroxyl radicals generated from 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH).

The antioxidant inhibition rates for uric acid and trolox were measured on addition of antioxidant reagents to the aqueous portion of the reaction mixture. Other comparative data were obtained by adjusting concentrations of the reduced substrates or concentrations of the AAPH peroxyl initiator.

Data were prepared for graphic presentation, and reaction rates were determined by linear regression using Kaleida-Graph software (Synergy Software, Reading, Pa.).

The inaugural test examined 166 methanolic extracts obtained from a selection of coral reef organisms and commensal microbes cultured on marine agar. Extracts included the marine sponges Paramyxilla n.sp. and Lissodendoryx isodicryalis; and the halophilic algae, Dampier Salt #1A.

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Title Antioxidant activity in extracts of marine organisms screened by microtiter plate assay
Language English
Licence Other
Landing Page https://devweb.dga.links.com.au/data/dataset/0dc3a3ec-3dee-4a1e-af84-dc42ac78948e
Contact Point
Australian Institute of Marine Science
adc@aims.gov.au
Geospatial Coverage GA1
Data Portal data.gov.au

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This dataset was originally found on data.gov.au "Antioxidant activity in extracts of marine organisms screened by microtiter plate assay". Please visit the source to access the original metadata of the dataset:
https://devweb.dga.links.com.au/data/dataset/antioxidant-activity-in-extracts-of-marine-organisms-screened-by-microtiter-plate-assay

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