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Extracellular phosphatase enzyme activity in reef waters of the central Great Barrier Reef

Created 24/06/2017

Updated 09/10/2017

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A fluorometric procedure was applied to measure changes in phosphatase activity in seawater flowing across reef flats at Bowl and Hopkinson Reefs on the central Great Barrier Reef. Seawater samples were collected at 50 m intervals across the reef flat during low tide, commencing at the reef front using a volume transport method and a time-distance-depth record was maintained allowing measurements to relate to the distance a water mass had flowed across the coral reef flat. Water samples were collected below the surface using a dip-scoop, filtered through 10 µm mesh and stored in the dark before being assayed.

Fluorescence was measured on 2 ml filtered (Nucleopore, 0.2 µm) and unfiltered subsamples on addition of 50 µl of a 5.0 mM solution of the enzyme substrate (4-methylumbelliferyl phosphate) and then at hourly intervals. An 8 hour period of incubation was required to determine statistically significant rates of hydrolysis from oligotrophic water samples.

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Title Extracellular phosphatase enzyme activity in reef waters of the central Great Barrier Reef
Language English
Licence Other
Landing Page https://devweb.dga.links.com.au/data/dataset/4b3f5043-016a-4415-828c-631a191e8036
Contact Point
Australian Institute of Marine Science
adc@aims.gov.au
Geospatial Coverage {"type": "Polygon", "coordinates": [[[147.206448, -18.562312], [147.183511, -18.562312], [147.183511, -18.538234], [147.206448, -18.538234], [147.206448, -18.562312]]]}
Data Portal data.gov.au

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This dataset was originally found on data.gov.au "Extracellular phosphatase enzyme activity in reef waters of the central Great Barrier Reef". Please visit the source to access the original metadata of the dataset:
https://devweb.dga.links.com.au/data/dataset/extracellular-phosphatase-enzyme-activity-in-reef-waters-of-the-central-great-barrier-reef