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Haemolymph protein in xanthid crabs from the Great Barrier Reef

Created 24/06/2017

Updated 09/10/2017

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Haemolymph was obtained from 26 xanthid crabs collected at low tide from Reef 21-145 and Laver's Cay on the Great Barrier Reef. A combinatin of protease inhibitors (5 mM EDTA, 1 µM pepstatin, 1 µM aprotonin and 100 µM phenylmethylsulfonyl fluoride) was added to the haemolymph. Measurements of equilibrium 3H-STX-binding, time courses of dissociation and association, and the pH-dependence of 3H-STX-binding was conducted on the crabs. Species were: Lophozozymus pictor (2), Liomera tristis (3), Chlorodiella nigra (2), Actaeodes tomentosus (2), Atergatis floridus (15), and Platypodia granulosa (2).

Rat-brain synaptosomes were prepared from postmortem rat-brain tissue and binding assays conducted. Tissue extracted from each crab tested for 3H-STX-binding activity was then assayed for paralytic shellfish toxin (PST) activity by rat synaptosome.

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Title Haemolymph protein in xanthid crabs from the Great Barrier Reef
Language English
Licence Other
Landing Page https://devweb.dga.links.com.au/data/dataset/9ee88998-8a42-4b4b-9580-fb9875659c4b
Contact Point
Australian Institute of Marine Science
adc@aims.gov.au
Geospatial Coverage {"type": "Point", "coordinates": [169.26472, -19.535]}
Data Portal data.gov.au