These data sets include Salinity, Carbon, Alkalinity, Oxygen and Nutrients (Silicate, Ammonium, Nitrite/Nitrate, Phosphate) analysed from samples collected as part of the Integrated Marine Observing Systems (IMOS) National Mooring Network -National Reference Station (NRS) field sampling. Nine Sites are included in the IMOS NRS field sampling stations. Maria Island (TAS), Kangaroo Island (SA), Esperance (WA), Rottnest Island (WA), Ningaloo (WA), Darwin (NT), Yongala (QLD), North Stradbroke Island (QLD) and Port Hacking 100 (NSW). Sampling at these sites is conducted by several members of IMOS NRS Biogeochemical project. Sampling at the sites began between 29/9/2008 and 8/11/2010 and is currently ongoing for 7 sites. Sampling ceased at Ningaloo and Esperance (WA) sites in Aug/Sept 2013. Sampling is conducted monthly (Esperance and Ningaloo were 3 monthly) off small vessels. Details including information on station locations, depths sampled, sample regime, sample types collected, and analyses can be found in the “IMOS National Reference Stations Biogeochemical Operations Manual” (see link below to latest version, plus link to previous versions and supplementary documentation). Historical data collected as part of CSIRO Coastal Monitoring program is available for three of the National Reference Station sites: Maria Island Coastal Station Data 1944-2008, Port Hacking 100m Coastal Station Data 1953-2010 and Rottnest Island Coastal Station Data 1951-2009. Additional information for this dataset may be available via the original MarLIN metadata entry: https://marlin.csiro.au/geonetwork/srv/eng/catalog.search#/metadata/fa93c66e-0e56-7e1d-e043-08114f8c1b76. The links to the metadata and access for these data is provided below. Data storage and access is planned to be interoperable with other national and international programs through the IMOS Infrastructure. Station metadata can be found through this resource. This dataset has been superseded by the "IMOS - Combined Biogeochemical parameters (reference stations)" collection that is available at https://catalogue-imos.aodn.org.au/geonetwork/srv/api/records/b442c3e8-3d30-48ad-b144-680afd848167 (this collection was removed from the AODN Portal in February 2022).