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Marine eukaryotes and bacteria, Antarctica

Created 24/06/2017

Updated 09/10/2017

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Glass slides were deployed at 12m and 18m3 at sites in McMurdo Sound (adjacent to McMurdo Station, Scott Base and Cape Armitage) for a period of 12 months to collect biofilm.

18S rDNA sequence information was obtained for representatives of each DGGE (denaturing gradient gel electrophoresis) band at each site and depth. This data was interrogated to phyla level: cnidaria, bryozoa, protozoa, dinoflagellates, arthropods, platyhelminths and annelids were present.

Density of diatoms observed by fluorescence microscopy for each site and depth were recorded (per 100 µm2). At least 6 different diatom morphotypes were identified based on size and morphology.

The slides were also examined by performing fluorescence in situ hybridization (FISH) reactions with bacteria-specific probes (Eub338), an archaea-specific probe (ARCH915) and 9 different bacterial group-specific probes.

Bacterial groups identified from the sequences were: Gammaproteobacteria, SRB, Planctomycetales, Alphaproteobacteria, Archaea, Betaproteobacteria, Actinobacteria and Firmicutes, Cytophaga /Flavobacteria of Bacteroidetes (CFB), Verrucomicrobia.

This study was the first to examine eukaryotic recruitment, potentially overlooked in visual surveys, using molecular techniques.

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Additional Info

Field Value
Title Marine eukaryotes and bacteria, Antarctica
Language English
Licence Other
Landing Page https://devweb.dga.links.com.au/data/dataset/9851d844-672b-407b-bce1-7373c6c3ed8b
Contact Point
Australian Institute of Marine Science
adc@aims.gov.au
Geospatial Coverage {"type": "Point", "coordinates": [166.6732, -77.863]}
Data Portal data.gov.au

Data Source

This dataset was originally found on data.gov.au "Marine eukaryotes and bacteria, Antarctica". Please visit the source to access the original metadata of the dataset:
https://devweb.dga.links.com.au/data/dataset/marine-eukaryotes-and-bacteria-antarctica

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