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Microbial communities in the sponge, Rhopaloeides odorabile, from the Great Barrier Reef

Created 12/03/2025

Updated 12/03/2025

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Specimens of the marine sponge Rhopaloeides odorabile (class Demospongiae; order Dictyoceratida; family Spongiidae) were collected from Davies Reef and Lizard Island on the Great Barrier Reef (3 from each location).Molecular techniques were employed to document the microbial diversity associated with the marine sponge Rhopaloeides odorabile.The phylogenetic affiliation of sponge-associated bacteria was assessed by 16S rRNA sequencing of cloned DNA fragments. Fluorescence in situ hybridization (FISH) was used to confirm the presence of the predominant groups indicated by 16S rDNA analysis. Novel media incorporating sponge extracts were used to isolate bacteria not previously recovered from this sponge.A radial cladogram was constructed showing the diversity of bacterial clone sequences from R. odorabile. Neighbor-joining phylogenetic trees were constructed from analyses of: 823 bp of 16S rRNA gene sequence included 34 clones for which unique sequence was obtained;709 bp of 16S rRNA gene sequence from clones clustering with the Actinobacteria;804 bp of 16S rRNA gene sequence from clones clustering within the predominantly g-Proteobacteria;811 bp of 16S rRNA gene sequence from clones clustering with green nonsulfur bacteria and d-Proteobacteria;305 bp of 16S rDNA sequence obtained from organisms cultured using sponge extract media.Branches were also found using the Fitch-Margoliash and maximum parsimony methods. The effects of using actinomycete-selective media supplemented with sponge extract on total number and the number of novel bacterial morphotypes isolated from R. odorabile were measured. Medium preparations were: marine agar 2216; starch-casein agar; Emerson agar; raffinose-histidine agar; M31 Casamino Acids agar; actinomycete isolation agar; yeast-malt extract agar; and glycerol-asparagine agar. The effects of stress, including copper exposure and elevated sea temperatures on the sponge microbial community were assessed. These impacts cause a distinct shift in the microbial community including a move away from symbiosis towards disease-causing microbes.Community structure included representatives of the Actinobacteria, low-GC Gram-positive bacteria, the b- and g-subdivisions of the Proteobacteria, Cytophaga/Flavobacterium, green sulfur bacteria, green nonsulfur bacteria, planctomycetes, and other sequence types with no known close relatives. The study aimed to investigate the diversity of the total bacterial community within the sponge R. odorabile.

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Field Value
Title Microbial communities in the sponge, Rhopaloeides odorabile, from the Great Barrier Reef
Language eng
Licence notspecified
Landing Page https://devweb.dga.links.com.au/data/dataset/8b3c9d4a-c20b-4aee-b62d-10bbe9eba55b
Contact Point
CSIRO Oceans & Atmosphere
adc@aims.gov.au
Reference Period 20/11/2017
Geospatial Coverage {"type": "Point", "coordinates": [147.641667, -18.825]}
Data Portal data.gov.au

Data Source

This dataset was originally found on data.gov.au "Microbial communities in the sponge, Rhopaloeides odorabile, from the Great Barrier Reef". Please visit the source to access the original metadata of the dataset:
https://devweb.dga.links.com.au/data/dataset/microbial-communities-in-the-sponge-rhopaloeides-odorabile-from-the-great-barrier-reef

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No duplicate datasets found.