Waters associated with the main reef complex at Lizard Island, North Queensland were studied during September 1977 as a component of the second "Lizard Island Metabolic Exchange on Reefs" (LIMER II) expedition. Two approaches were taken:a) Nutrient Transects; water sampling for biological and nutrient analyses was carried out along fixed transects (one leeward reef, one windward reef) on which radar-reflecting buoys were set up marking boundaries between major benthic zones and b) Hydrology Transects; temperature, salinity and oxygen data was determined to describe water structure.
a) Nutrient Transects Sampling stations on the windward reef were Af-Gf with an offshore station (Hf) approximately 1km in front of the reef face. Station Af was approximately 50m in front of the reef crest in a water depth of about 20m. Stations Bf and Cf were not marked by buoys but were about 15m on either side of the reef crest. Station Bf was in a water depth of about 7m. The remaining stations (Df-Gf ) were marked by buoys and positioned across the 800m wide reef flat.Sampling stations on the leeward reef were Hb, Ab-Fb. Station Hb was about 60m in front of the reef margin in lagoonal waters about 2.5m deep. Station Ab was immediately in front of the reef margin in water about 1m deep. Remaining stations (Cb-Fb) were positioned across about 700m of the leeward reef flat. Depths and the typical profiles were obtained from a series of runs across the transects with a depth sounder. The tidal datum was determined from the tidal plane for Lizard Island in reference to tidal predictions for Cairns (the standard port for Lizard Island). Depth at each station was calculated from the datum point establishment for the station and the predicted tide height for Lizard Island.Water SamplingFluorescein or a curtain drogue (at night) was placed in the water at the front of a transect. For the windward reef, transects began at Station Af. Leeward reef transects began at station Hb. Samples of water were taken by 2/3 filling of nalgene plastic containers (40l) previously treated with flowing seawater to remove traces of plasticizer. The plastic bins were placed in the water alongside the boat and washed several times before being filled. A second boat ferried water samples back to the laboratory ensuring that samples were not stored for more than 2h before analysis, thus minimizing temperature variation, and chemical and biological changes. Samples were taken at station Hf, 1km offshore, at about the same time that sampling of windward reef transects commenced. On return to the laboratory, water samples were gently mixed and subsamples removed for the various analyses. The time and position of sampling was recorded on a large aerial photograph by observers on a hill above the reef complex. If water flowed away from the line of buoys, samples were taken at boundaries of the biotic zonation marked by the buoys.At night, these positions were determined by the onshore observers using the aerial photograph and radar positions of the sampling boat and marker buoys. A string of 810 crushed aluminium beer cans was found to give a better radar reflection than vertical aluminium plates at right angles because the latter did not maintain reflection orientation in strong winds (>7m/sec) or at low tide. Samples were taken alongside the centre of the patch of fluorescein during the day. Daytime deployment of the curtain drogue showed that it moved, on average, about 1.3 times faster than the fluorescein; however, night time samples were taken as the drogue reached each appropriate point. On two occasions, standing water samples were collected across the windward reef transect independent of water mass flow. On the leeward reef, two transects were carried out in which a volume of water was enclosed in a large plastic bag at station Ab, the bag was allowed to drift with the water current, and water samples were taken from the bag and the traversing seawater at each station (referred to as "Bgd" in data tables).b) Hydrology TransectsPreliminary studies of movement, horizontal and vertical mixing processes, and light transmission were made in the Lizard Island reef waters. Some study was carried out at stations on the Nutrient Transects of the windward and leeward reefs, while others were at discrete stations across the reef system; these stations being established to determine characteristics of particular hydrological events. Temperature, salinity, oxygen concentration and light transmission were determined.
