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Sampling of planktonic microorganisms for metagenomic, metaproteomic and metatranscriptomic analyses

Created 24/06/2017

Updated 24/06/2017

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Large volumes of water (200 - 500 L) were filtered and fractionated by size for various planktonic components: eukaryotic phytoplankton, prokaryotic picoplankton, and marine viruses. Sample sites were chosen to generate the widest diversity, and included planktonic blooms, oligotrophic zones, small polynyas near sea ice, nearshore areas, and Antarctic bottom water from coastal, canyon and deepwater areas. Half of each sample will be used for DNA library construction, and the other half will be used for meta-proteomic analysis. Random shotgun sequencing of the marine genomic libraries should produce a metagenomic snapshot of planktonic life in a variety of marine habitats.

This work was completed as part of ASAC project 2899 (ASAC_2899).

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Title Sampling of planktonic microorganisms for metagenomic, metaproteomic and metatranscriptomic analyses
Language English
Licence Other
Landing Page https://devweb.dga.links.com.au/data/dataset/124d8f7f-c18b-40a8-bbe5-c22ef47ac118
Contact Point
Australian Antarctic Division
metadata@aad.gov.au
Geospatial Coverage {"type": "Polygon", "coordinates": [[[140.0, -67.0], [150.0, -67.0], [150.0, -46.0], [140.0, -46.0], [140.0, -67.0]]]}
Data Portal data.gov.au