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Saxitoxin in octopi from Cooke Point, Port Hedland, Western Australia

Created 24/06/2017

Updated 09/10/2017

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Octopus (Abdopus) sp. 5 were collected from a reef flat at Cooke Point, Port Hedland in northern Western Australia. The octopi had a body size of between 5-6 cm and tentacle span of between 20 and 30 cm.Specimens were euthanased by rapid decapitation, stored at -20°C and shipped frozen to the Australian Institute of Marine Science in Townsville. Three legs from each of the four individuals were pooled for toxin extraction. Each of the four samples was extracted with 80% ethanol, homogenised, sonicated three times for 10 minutes, then clarified by centrifugation. This process was repeated two additional times with fresh extraction solvent. For each of the four extracts, the three supernatants generated were pooled, filtered through 0.2 mm nylon filters and lyophilised. Dried extracts were reconstituted in 0.05 M acetic acid and passed through a centrifugal ultra-filter and the ultra filtrates then used for radio-receptor assay and preliminary chemical analysis.Quantitation of STX concentration equivalents (STXeq) with [³H]-STX radio-receptor assays using saxiphilin and voltage gated sodium channel, was performed and octopus extracts were assayed in triplicate for inhibition of STX binding in the sodium channel and saxiphilin assay.All sample extracts which competed with [³H]-STX in the radio-receptor assays were analysed by LC-FLD for STX, decarbamoylSTX, neoSTX, 11-hydroxysulphate STXs (GTX1-6), N-sulfocarbamoyl-11-hydroxy-sulphate STXs (C1-4), hydroxybenzoate STXs and TTX.Identification of suspect toxin peaks was undertaken by mass spectrometric analysis of separated toxins using liquid chromatography-mass spectrometry (LC-MS) performed in single ion monitoring (SIM) mode.

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Title Saxitoxin in octopi from Cooke Point, Port Hedland, Western Australia
Language English
Licence Other
Landing Page https://devweb.dga.links.com.au/data/dataset/3e794f35-df8e-4726-9671-e88f6787bad9
Contact Point
Australian Institute of Marine Science
adc@aims.gov.au
Geospatial Coverage {"type": "Point", "coordinates": [118.6408, -20.29782]}
Data Portal data.gov.au

Data Source

This dataset was originally found on data.gov.au "Saxitoxin in octopi from Cooke Point, Port Hedland, Western Australia". Please visit the source to access the original metadata of the dataset:
https://devweb.dga.links.com.au/data/dataset/saxitoxin-in-octopi-from-cooke-point-port-hedland-western-australia

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