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Uptake of dissolved free amino acids by larvae of the crown-of-thorns starfish, Acanthaster planci, from the Great Barrier Reef

Created 12/03/2025

Updated 12/03/2025

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Early brachiolaria larvae (6 days old) were reared at the Australian Institute of Marine Science for use in dissolved organic matter uptake experiments. Three dissolved free amino acids (substrates) were chosen for the experiments: alanine (neutral); glutamic acid (acidic); and arginine (basic). Between 120 and 240 larvae were placed in individual scintillation vials containing filtered seawater and maintained at 29.5°C. Experiments were run using filtered seawater with only one substrate. Different amounts of substrates were added to vials with or with larvae, as well as vials with formalin killed larvae. Triplicate 500 µl samples were withdrawn from each vial initially and then after incubation for 20, 40 and 60 minutes. Samples were passed through a 100 µm mesh screen to remove larvae and frozen in a micro-centrifuge tube for later DFAA analysis using the pre-column derivatization method with HPLC. This research was undertaken to investigate the ability of early brachiolaria larvae of the crown-of-thorns starfish, Acanthaster planci, to take up dissolved organic matter (DOM) in the form of dissolved free amino acids (DFAA).

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Title Uptake of dissolved free amino acids by larvae of the crown-of-thorns starfish, Acanthaster planci, from the Great Barrier Reef
Language eng
Licence notspecified
Landing Page https://devweb.dga.links.com.au/data/dataset/e122200c-79b9-4701-bd46-7b249602bf06
Contact Point
CSIRO Oceans & Atmosphere
reception@aims.gov.au
Reference Period 20/11/2017
Geospatial Coverage {"type": "Point", "coordinates": [147.056089, -19.268221]}
Data Portal data.gov.au

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This dataset was originally found on data.gov.au "Uptake of dissolved free amino acids by larvae of the crown-of-thorns starfish, Acanthaster planci, from the Great Barrier Reef". Please visit the source to access the original metadata of the dataset:
https://devweb.dga.links.com.au/data/dataset/uptake-of-dissolved-free-amino-acids-by-larvae-of-the-crown-of-thorns-starfish-acanthaster-plan1

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