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UV-absorbing pigments in eye tissues of fish from the Great Barrier Reef

Created 24/06/2017

Updated 09/10/2017

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Ocular tissues from 52 species (19 families) of tropical marine fish were examined for levels of UV-absorbing pigments in the spectral region 300-380 nm.

Specimens of mackerels, tuna and marlin were caught by surface trolling in Bowling Green and Upstart Bays; all other fish were caught by hand-line or spearfishing at Grub, Hunt and Marion Reefs. The eyes were removed and frozen immediately at -20°C. Lens ocular tissues were excised from defrosted samples, crushed and extracts formed by steeping in 5ml methanol. Visible-u.v. absorption spectra from these extracts were recorded. Solid protein biomass was determined from the solid tissue residues. Mycosporine-like amino acids (Asterina-330, palythene, palythine and palythinol) were separated and quantified (nmol/mg protein) using reverse-phase isocratic high-performance liquid chromatography (HPLC).

The efficiency of methanol extraction

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Title UV-absorbing pigments in eye tissues of fish from the Great Barrier Reef
Language English
Licence Other
Landing Page https://devweb.dga.links.com.au/data/dataset/5933d64e-55f2-4403-a2f4-b0a745060882
Contact Point
Australian Institute of Marine Science
adc@aims.gov.au
Geospatial Coverage {"type": "Polygon", "coordinates": [[[147.20917, -20.24283], [152.38333, -20.24283], [152.38333, -18.63333], [147.20917, -18.63333], [147.20917, -20.24283]]]}
Data Portal data.gov.au

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This dataset was originally found on data.gov.au "UV-absorbing pigments in eye tissues of fish from the Great Barrier Reef". Please visit the source to access the original metadata of the dataset:
https://devweb.dga.links.com.au/data/dataset/uv-absorbing-pigments-in-eye-tissues-of-fish-from-the-great-barrier-reef

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